Biotechnology
- Lecture Notes, Study Materials and Important questions answers

Subject : Biotechnology

Recombinant DNA Technology

1. Tools of rDNA technology - Answer (click here)
2. Restriction Fragment Length Polymorphism (RFLP) - Answer (click here)
3. Vectors: Vehicles for cloning - Answer (click here)
4. Plasmids - Answer (click here)
5. Vectors based on bacteriophages - Answer (click here)
6. Making rDNA - Answer (click here)
7. Introduction of rDNA into host cells - Recombinant DNA Technology - Answer (click here)
8. Identification of Recombinants - Recombinant DNA Technology - Answer (click here)
9. Polymerase Chain Reaction (PCR) - Answer (click here)
10. Hybridisation Techniques - Recombinant DNA Technology - Answer (click here)
11. DNA Library - Answer (click here)
12. DNA Sequencing - Answer (click here)
13. Site-directed Mutagenesis - Answer (click here)

Protein Structure And Engineering

1. Introduction to the World of Proteins - Answer (click here)
2. 3-D Shape of Proteins - Answer (click here)
3. Structure-Function relationship in Proteins - Answer (click here)
4. Chymotrypsin, a proteolytic enzyme - Answer (click here)
5. Molecular Disease- Sickle cell anaemia - Answer (click here)
6. Protein Finger printing- Peptide Mapping - Answer (click here)
7. 2-D Gel Electrophoresis - Answer (click here)
8. Purification of Proteins - Answer (click here)
9. Characterisation of Proteins - Answer (click here)
10. Protein Based Products - Answer (click here)
11. Designing Proteins (Protein Engineering) - Answer (click here)

Genomics and Bioinformatics

1. Genomics and Bioinformatics - Answer (click here)
2. Genome Sequencing Projects - Answer (click here)
3. Gene prediction and counting - Answer (click here)
4. Genome Similarity, SNPs and Comparative Genomics - Answer (click here)
5. Functional Genomics - Answer (click here)
6. Fluorescence in situ hybridization - Answer (click here)
7. Microarray Technology - Answer (click here)
8. Proteomics and Types of Proteomics - Answer (click here)
9. History of Bioinformatics - Answer (click here)
10. Sequences and nomenclature - Bioinformatics - Answer (click here)
11. Different types of sequences - Bioinformatics - Answer (click here)
12. Information Sources - Bioinformatics - Answer (click here)
13. Analysis using Bioinformatics tools - Answer (click here)

Microbial Cell Culture and its Applications

1. Microbial Cell Culture and its Applications - Answer (click here)
2. Microbial culture techniques - Answer (click here)
3. Measurement and kinetics of microbial growth - Answer (click here)
4. Scale-up of microbial processes - Answer (click here)
5. Isolation of microbial products - Answer (click here)
6. Strain isolation, improvement and preservation - Answer (click here)
7. Applications of microbial culture technology - Answer (click here)
8. Biosafety issues in Microbial Technology - Answer (click here)

Plant Cell Culture and Applications

1. Plant Cell Culture and Applications - Answer (click here)
2. Cell and Tissue Culture Techniques - Answer (click here)
3. Applications of Cell and Tissue Culture - Answer (click here)
4. Gene transfer methods in plants - Answer (click here)
5. Transgenic plants with beneficial traits - Answer (click here)
6. Biotic and Abiotic stress tolerance - Answer (click here)
7. Biosafety in Plant Genetic Engineering - Answer (click here)

Animal Cell Culture and Applications

1. Animal Cell Culture and Applications - Answer (click here)
2. Animal Cell Culture Techniques - Answer (click here)
3. Characterization of Cell Lines - Answer (click here)
4. Methods of Gene Delivery into Cells - Answer (click here)
5. Scale-up of Animal Culture Process - Answer (click here)
6. Applications of Animal Cell culture - Answer (click here)
7. Stem Cell Technology - Answer (click here)
8. Tissue engineering - Answer (click here)