Thursday, 10 May 2018

Basic Concept of Biotechnology - Lecture Notes, Study Materials and Important questions answers



Basic Concept of Biotechnology
- Lecture Notes, Study Materials and Important questions answers




Subject : Basic Concept of Biotechnology

Biomolecules

  1. Biomolecules - Answer (click here)
  2. Carbohydrates - Answer (click here)
  3. Classification of Carbohydrates - Answer (click here)
  4. Structure of Monosaccharide’s - Answer (click here)
  5. Structure of Di Saccharides and Polysaccharides - Answer (click here)
  6. Importance of carbohydrates - Answer (click here)
  7. Rare sugars - Answer (click here)
  8. Nucleic Acids - Answer (click here)
  9. Structure of Nucleic Acids - Answer (click here)
  10. Watson and Crick’s double helix structure of DNA - Answer (click here)
  11. Tautomerism - Answer (click here)
  12. Nucleosides and nucleotides - Answer (click here)
  13. Proteins - Biomolecules - Answer (click here)
  14. Amino Acids and Classification of Amino Acids - Answer (click here)
  15. Non-proteinogenic amino acids and its role - Answer (click here)
  16. Classification of Proteins - Answer (click here)
  17. Structure of Proteins - Answer (click here)
  18. Biological Importance of Proteins - Answer (click here)
  19. Lipids and Classification of Lipids - Answer (click here)
  20. Structure of lipids - Answer (click here)
  21. Fatty Acids - Answer (click here)
  22. Triacylglycerols - Answer (click here)
  23. Phospholipids - Answer (click here)
  24. Sterols and sterol esters - Answer (click here)

Computer Applications and Biostatistics

  1. Computer Applications and Biostatistics - Answer (click here)
  2. Information and computer technology - Answer (click here)
  3. Biostatistics - Answer (click here)
  4. Types of Data - Biostatistics - Answer (click here)
  5. Measures of Central Tendencies - Biostatistics - Answer (click here)
  6. Types of Distribution - Biostatistics - Answer (click here)
  7. Applications of Standard Error of Mean - Answer (click here)
  8. Confidence Interval (CI) - Answer (click here)
  9. Null Hypothesis - Answer (click here)
  10. Level of Significance - Answer (click here)
  11. Outliers - Answer (click here)
  12. One tailed and two tailed Test - Answer (click here)
  13. Importance of Sample Size Determination - Answer (click here)
  14. Factors Influencing Sample Size Include - Answer (click here)
  15. Sample Size Determination and Variance Estimate - Answer (click here)
  16. How to Choose an Appropriate Statistical Test - Answer (click here)
  17. Non-parametric tests used for analysis of different types of data - Answer (click here)
  18. Common problems faced by researcher in any trial and how to address them - Answer (click here)
  19. Biological data and its diversity - Answer (click here)
  20. Application of computer and statistics in biology - Answer (click here)

Macromolecules and Analytical Techniques

  1. Macromolecules and Analytical Techniques - Answer (click here)
  2. Macromolecules - Answer (click here)
  3. Centrifugation - Analysis of biomolecules - Answer (click here)
  4. Chromatography: Types, Principle, Application - Analysis of biomolecules - Answer (click here)
  5. Electrophoresis - Analysis of biomolecules - Answer (click here)
  6. Spectroscopy - Analysis of biomolecules - Answer (click here)
  7. UV Visible spectroscopy - Analysis of biomolecules - Answer (click here)
  8. Infra Red Spectropscopy (IR spectroscopy) - Analysis of biomolecules - Answer (click here)
  9. Fluorometry - Analysis of biomolecules - Answer (click here)
  10. Mass Spectrometry - Analysis of biomolecules - Answer (click here)
  11. Nuclear Magnetic Resonance (NMR) spectroscopy - Analysis of biomolecules - Answer (click here)

Plant Molecular Farming A Promising Stratergy in Biotechnology

  1. Plant Molecular Farming: A Promising Stratergy in Biotechnology - Answer (click here)
  2. Plants as Expression Systems - Answer (click here)
  3. Types of Biomolecules and recent examples - Answer (click here)
  4. Plant made pharmaceuticals (PMPs) - Answer (click here)
  5. Plant made nutritional compounds (PMNs) - Answer (click here)
  6. Plant made vaccines (PMVs) - Answer (click here)
  7. Plant made plastics (PMPs) - Answer (click here)
  8. Plant made industrial compounds (PMIs) - Answer (click here)
  9. The plant endomembrane system - Answer (click here)
  10. Subcellular Targeting of proteins in plants - Answer (click here)
  11. Different plant species as platform for molecular farming - Answer (click here)
  12. Plant Transformation - Answer (click here)
  13. Optimization of transgene expression in host plant - Answer (click here)
  14. Downstream processing of recombinant proteins - Answer (click here)
  15. Use of affinity tags for purification - Answer (click here)
  16. Use of non-chromatographic methods - Answer (click here)
  17. Biosafety and regulatory issues - Answer (click here)

Plant Transgenics Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants

  1. Plant Transgenics: Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants - Answer (click here)
  2. The Power of Biotechnology - Answer (click here)
  3. Strategies for Competitiveness - Biotechnology - Answer (click here)
  4. Tools of Genetic Engineering in Plants - Answer (click here)
  5. DNA Delivery Systems - Plant Transgenics - Answer (click here)
  6. The Selection and Analysis of Transformants - Plant Transgenics - Answer (click here)
  7. Modification of regulatory elements of marker genes - Plant Transgenics - Answer (click here)
  8. More ‘Friendly’ Selectable Markers: the Positive Selection Method - Plant Transgenics - Answer (click here)
  9. Biotic Stress - Answer (click here)
  10. Virus Resistance - Answer (click here)
  11. RNAi Technology - Answer (click here)
  12. Fungal Resistance - Answer (click here)
  13. Bacterial Resistance - Answer (click here)
  14. Nematode Resistance - Answer (click here)

Animal Biotechnology

  1. Animal Biotechnology - Answer (click here)
  2. Cell Culture - Animal Biotechnology - Answer (click here)
  3. Criteria for Subculture - Animal Biotechnology - Answer (click here)
  4. Culture Conditions and Mammalian Cell - Animal Biotechnology - Answer (click here)
  5. Aseptic Techniques - Animal Biotechnology - Answer (click here)
  6. Cryopreservation Medium - Answer (click here)
  7. Protocol for Cryopreserving Cultured Cells - Answer (click here)
  8. Protocol for Thawing Frozen Cells - Answer (click here)
  9. Biological Contamination - Answer (click here)
  10. Protocol for Microbial Decontamination - Answer (click here)
  11. Protocol for Treating Mycoplasma contaminated Cell Cultures with BM Cyclin - Answer (click here)
  12. Cell Viability (quantitation and cytotoxicity) - Answer (click here)
  13. Factors to Consider When Choosing a Cell Viability Assay - Answer (click here)
  14. Cell Viability Assays that Measure ATP and Metabolic Capacity - Answer (click here)
  15. Cytotoxicity Assays: Determining the Number of Live and Dead Cells - Answer (click here)
  16. Trypan Blue - Answer (click here)
  17. Concentrating Cells - Answer (click here)
  18. Cell Proliferation - Animal Biotechnology - Answer (click here)
  19. Cell Cycle - Animal Biotechnology - Answer (click here)
  20. Cytotoxicity - Answer (click here)
  21. Applications of cell culture - Answer (click here)
  22. What are stem cells, and why are they important? - Answer (click here)
  23. What are the unique properties of all stem cells? - Answer (click here)
  24. Embryonic stem cells grown in the laboratory - Answer (click here)
  25. Similarities and Differences between Embryonic and Adult stem cells - Answer (click here)
  26. Potential uses of human stem cells and the obstacles - Answer (click here)
  27. Genetic manipulation of animals (animal models in research) - Answer (click here)
  28. Animal Breeding and Transgenic Animals - Answer (click here)
  29. Methods of genetic manipulation in animals - Answer (click here)
  30. A brief history of cloning - Answer (click here)
  31. Cloning - Answer (click here)
  32. Use of Animals in Research - Answer (click here)
  33. Improving Agricultural Products with Transgenics - Answer (click here)
  34. Genetically Engineered Insulin - Answer (click here)
  35. Gene Therapy - Answer (click here)
  36. Monoclonal Antibodies - Answer (click here)
  37. Economic impact in developing countries - Animal Biotechnology - Answer (click here)
  38. Ethical Issues(biosafety and regulatory issues) - Animal Biotechnology - Answer (click here)

Medical Biotechnology

  1. Medical Biotechnology - Answer (click here)
  2. History of Medical Biotechnology - Answer (click here)
  3. Human Genome Project and its influence on medical biotechnology - Answer (click here)
  4. Detecting Genetic Diseases - Answer (click here)
  5. Biotech in the Hospital - Answer (click here)
  6. Role of biotechnology in diagnostics and Therapeutics - Answer (click here)
  7. Role of biotechnology in developing vaccines - Answer (click here)
  8. Treatments developed with the aid of biotechnology - Answer (click here)
  9. Other Applications of Biotechnology in Medical field - Answer (click here)
  10. Designer Babies - Medical Biotechnology - Answer (click here)
  11. New Biotech Breakthroughs about to change Medicine - Answer (click here)

Tools and Techniques in Biotechnology

  1. Tools and Techniques in Biotechnology - Answer (click here)
  2. Basic Tools in the Biotechnology - Answer (click here)
  3. Measurement of Volume - Tools used in Biotechnology - Answer (click here)
  4. Measurement of Weight - Tools used in Biotechnology - Answer (click here)
  5. Measurement of pH - Tools used in Biotechnology - Answer (click here)
  6. Measurement of light - Tools used in Biotechnology - Answer (click here)
  7. Solution Preparation - Tools used in Biotechnology - Answer (click here)
  8. Microbiological techniques - Tools used in Biotechnology - Answer (click here)
  9. Preparation of biological samples for analysis - Tools used in Biotechnology - Answer (click here)
  10. Separation of macromolecules - Tools used in Biotechnology - Answer (click here)
  11. Manipulation of Nucleic Acids - Tools used in Biotechnology - Answer (click here)
  12. Techniques of Biotechnology and Innovations - Answer (click here)
  13. Isolation of Nucleic Acid (DNA) - Techniques of Biotechnology and Innovations - Answer (click here)
  14. Plasmid Isolation - Techniques of Biotechnology and Innovations - Answer (click here)
  15. RNA Isolation and mRNA Purification - Techniques of Biotechnology and Innovations - Answer (click here)
  16. Gene Isolation - Techniques of Biotechnology and Innovations - Answer (click here)
  17. Molecular Mapping - Techniques of Biotechnology and Innovations - Answer (click here)
  18. Genomic DNA Library Construction - Techniques of Biotechnology and Innovations - Answer (click here)
  19. Transformation Techniques - Techniques of Biotechnology and Innovations - Answer (click here)
  20. Direct Gene Transformation - Techniques of Biotechnology and Innovations - Answer (click here)
  21. Labeling Methods - Techniques of Biotechnology and Innovations - Answer (click here)
  22. Southern Blotting Technique - Techniques of Biotechnology and Innovations - Answer (click here)
  23. In Situ Hybridization (ISH) - Techniques of Biotechnology and Innovations - Answer (click here)
  24. Polymerase Chain Reaction(PCR) - Techniques of Biotechnology and Innovations - Answer (click here)
  25. Fluorescence In Situ Hybridization (FISH) - Techniques of Biotechnology and Innovations - Answer (click here)
  26. DNA Sequencing Techniques - Techniques of Biotechnology and Innovations - Answer (click here)
  27. Microarray - Techniques of Biotechnology and Innovations - Answer (click here)
  28. Next Generation Sequencing (NGS) - Techniques of Biotechnology and Innovations - Answer (click here)

Antibiotics Microbial Sources Production and Optimization

  1. Antibiotics - Answer (click here)
  2. Antibacterial agents - Answer (click here)
  3. Actinomycetes - Answer (click here)
  4. Marine actinomycetes - Answer (click here)
  5. Streptomyces - Answer (click here)
  6. Identification of actinomycetes - Answer (click here)
  7. Metabolite production by marine Streptomyces - Answer (click here)
  8. Antagonistic marine actinomycetes in Indian peninsula - Answer (click here)
  9. Optimization of fermentation conditions - Answer (click here)

Biotechnology in Forensic Sciences

  1. History of Forensic Genetic Typing - Answer (click here)
  2. Introduction - Biotechnology in Forensic Sciences - Answer (click here)
  3. Restriction Fragment Length Polymorphism (RFLP) - Answer (click here)
  4. Amplified Fragment Length Polymorphism (AMP-FLP) - Answer (click here)
  5. Polymerase Chain Reaction (PCR) - Biotechnology in Forensic Sciences - Answer (click here)
  6. Short Tandem Repeats (STRs) - Biotechnology in Forensic Sciences - Answer (click here)
  7. Y-Short Tandem Repeats - Answer (click here)
  8. X-chromosomal short tandem repeats (ChrX STRs) - Answer (click here)
  9. mtDNA in forensic science - Answer (click here)
  10. Microbial Forensics - Answer (click here)
  11. Non-human DNA Forensics - Answer (click here)
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